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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: MBD3L2 Interacts with MBD3 and Components of the NuRD Complex and Can Oppose MBD2-MeCP1-mediated Methylation Silencing
doi: 10.1074/jbc.m413492200
Figure Lengend Snippet: FIG. 4. Two-hybrid interactions and in vitro binding of MBD3L2 and MBD3. A, yeast two-hybrid assay to dem- onstrate interactions between MBD2, MBD3, and MBD3L2. The coding se- quences were cloned into either the GAL4 activation domain (GAL4-AD) vector or the GAL4 DNA binding domain (GAL4- BD) vector. For MBD2, the long and short forms, MBD2a and MBD2b, and the tes- tis-specific form MBD2t were used. The dashes indicate empty control vectors. Colonies were grown on SD/-Ade/-His/- Leu/-Trp plates. B, interaction of GST- tagged MBD3L2 or its deletion constructs with His-tagged MBD3 or MBD2b. The GST-tagged proteins were adsorbed onto glutathione-agarose beads. After exten- sive washing, bound proteins were eluted, separated on SDS-polyacylamide gels, transferred to a nylon membrane, and re- acted with an anti-His antibody. His- tagged MBD2b interacts only weakly with MBD3L2 but much more strongly with MBD3. C, interaction of GST-tagged MBD3 or its deletion constructs with FLAG-tagged MBD3L2 or His-tagged MBD2b.
Article Snippet: The membranes were incubated with
Techniques: In Vitro, Binding Assay, Y2H Assay, Clone Assay, Activation Assay, Plasmid Preparation, Control, Construct, Membrane